The lipoxygenase gene AfLOX4 of Amorpha fruticosa L. is a potential regulator of drought stress tolerance pathways under saline and alkaline conditions

Abstract Lipoxygenase (LOX, EC1.13.11.12) catalyzes the oxygenation of polyunsaturated fatty acids and further generates oxylipins via enzymatic or non-enzymatic pathways, thus participating in regulation growth development, stress response, other processes. The objective this research was to characterize function evaluate mechanism lipoxygenase gene AfLOX4 ’s role enhancing drought tolerance. Transcriptomic data were generated analyzed for LOX gene, under different concentrations (polyethylene glycol-6000) (PEG6000) abscisic acid (ABA) treatment, determine their effects on expression its plant physiology. localization protein detected through protoplast transformation. Amorpha fruticosa tissues determined by quantitative real-time PCR (qRT-PCR). Six genes A. that showed an upregulated pattern found transcriptomic analysis named AfLOX1 ~ 6. localized cytoplasm. expressed stems, roots, flowers, leaves L., level highest leaves. increased with prolongation time stresses. After ABA germination rate tobacco lines overexpressing significantly increased, while NaHCO 3 stress, tolerance plants higher than wild-type tobacco. overexpression natural method had a survival recovery experiment, improved This study reveals resisting lays solid theoretical foundation genetic improvement L. varieties, as well resource utilization.